Detection of anti-Toxoplasma antibodies in Blood Donors in Khartoum State

Abstract

The study was a descriptive cross sectional, that was conducted at the International laboratory Health of Khartoum State. Following informed consent, samples were collected at voluntary counseling and testing centers blood bank in Khartoum, Omdurman and Khartoum North teaching Hospitals. The study was done during 2011-2013. The study involved 534 samples taken from the blood donors; the study has investigated Toxaplasma gonadii by latex agglutination test, in addition to assessment of levels of IgG and IgM using the ELISA test. The area from which study samples were collected included Khartoum with 299(56%) samples, Omdurman with 118(22.1%) samples and Khartoum North with 117(21.9%) samples. Positive results were detected in 235(44%), 218(40.8%), 187(35%) by using latex agglutination test, ELISA for IgG and IgM levels respectively. All samples were examined by latex agglutination, ELISA IgG and IgM test for the presence of anti-Toxoplasma antibodies. The study population was divided in to7 age group (18-25), (26-30), (31-35), (36-40), (41-45), (46-50), (51-56) age distribution of the study groups with the latex agglutination test, ELISA IgG and IgM test. The highest rate (36%) was reported amang the age group18-25 years, but the lowest rate1.3% was reported amang the age group 51-56 years. The latex test, IgG and IgM tests were significant. The study showed that drinking non boiling milk found to be statistically significant in latex test and ELISA. IgM(P = 0.002),( P = 0.049) and not significant in ELISA IgG (P = 0.09). Contact with domestic animals was found to be non-significant in the transmission cycle. Eating uncooked or partially cooked meat has been shown to be of great importance in the transmission cycle.