Investigation of Phytochemicals From Parsley Fixed Oil by GC-MS and Antimicrobial Potency of the Oil

Abstract

In the present study, the fixed oil of Petroselinum crispum (Parsely) was extracted from seeds.The oil was analyzed by gas chromatography – mass spectrometry (GC-MS) and 62 components were identified .Major constituents were: Apiol (36.44%), 9-octadecenoic acid (Z)-, methyl ester (27.17%), 1, 3-benzodioxole, 4-methoxy-6-(2-propenyl) - (12.12%), 9, 12-octadecadienoic acid (Z, Z)- methyl ester (8.32%), hexadecanoic acid methyl ester (4.08%) and benzene, 1, 2, 3-trimethoxy-5-(2-propenyl) (2.09%). The oil was screened for antibacterial and antifungal activities using five standard human pathogens. Agar well diffusion technique was used to assess the antimicrobial activity of the oil against two Gram positive (Bacillus subtili and Staphylococcus aureus), two Gram negative (Escherichia coli and Pseudomonas aeroginosa) bacteria and one fungal species (Candida albicans).The results indicated that Petroselinum crispum oil possess bioactive compounds having significant antimicrobial properties.