Immunohistochemical Detection of P63 in Prostate Tumors among Sudanese Patients

Abstract

This hospital based analytical retrospective case control study conducted at Radiation / Isotope Center (RICK) and Omdurman hospital and Sudan University of Science and Technology- college of medical laboratory science during the period from August 2016 to January 2017. The study aimed to detect the p63 in prostate tumors using immunohistochemistry. Forty paraffin blocks were collected from patients previously diagnosed as prostate tumor, 20 (50%) samples were malignant and the other 20 (50%) samples were benign. The paraffin blocks were cut by rotary microtome then stained by immunohistochemistry method ( New indirect technique). The data obtained was analyzed using SPSS program version 20, mean, frequency and chi square test were calculated. The age of patients range between 50 to 90 years with mean age of 71 years. The study revealed that most patients were more than 65 years representing 28 (70%) and the remaining 12 (30%) were less than 65 years. The grade of tumors showed grade I in 3 (15%) samples, grade II in 3 (15%) samples, grade III in 4 (20%) samples, grade IV in 7 (35%) samples and grade V in 3 (15%) samples. Positive expression of p63 was found in (11/20) in malignant samples, and (9/20) samples showed negative expression, while in benign samples (15/20)showed positive expression, and (5/20) samples showed negative expression of p63, this result showed insignificant association between p63 expression and prostate tumors (P.value =0.185). The study concluded that expression of p63 is not associated with histopathological diagnosis of prostate tumor.