Frequency of Common Bacterial Uro ‫ـ‬pathogens and Their Susceptibility Patterns among Haemodialysis patients in Ahmed Gasim Hospital, Khartoum State

Abstract

This study was carried out in Ahmed Gassim Hospital, Khartoum State during the period from April to June 2012. A total of 100 mid-stream urine specimens were collected, they were cultured on Cystiene Lysine Electrolytes Deficiency (CLED) agar. The identification of uro-pathogens was done according to Chessbrough (2007) which involved colonial morphology, Gram stain and biochemical identification, using catalase test, coagulase test, DNase test, mannitol salt agar, indole test, urease test, citrate test, MR test, VP test, motility test, and kligler iron agar. Modified Kirby ‫ـ‬Bauer disc diffusion method was used to study the susceptibility patterns of isolated bacteria against the following antibiotics: amikacin, ampicillin, cotrimoxazole, ceftizoxime, cefotaxime, chloramphenicol, ciprofloxacin, gatifloxacin, cephalexin, sparfloxcin, norfloxacin and tetracycline. 56(56%) from all urine culture were showed significant bacterial growth and 44 (44%) showed no or insignificant growth. According to the scheme of Chessbrough) 2007(, six different type of bacteria were isolated and identified, the most dominant species was Escherichia coli 23 (41%), followed by Staphylococcus aureus21 (37.5%), Proteus spp. 5(9%), Klebsiella spp. 3(5.3%), Staphylococcus epidermidis and Pseudomonas spp. were represented2 (3.6%). All isolates 6 (100%) were resistant to ceftizoxime. and most of them 5(83%) resistant to cephalexin. All isolates 6 (100%) were sensitive to amikacin, chloramphenicol, and 5(83%) of them were sensitive to gatifoxacin while tetracycline and sparfloxcin were sensitive only to Staphylococcus spp. the result showed that the E.coli and Pseudomonas spp. were resistant to the most used antibiotic, they were sensitive only for 4 (30%) agents out of 12 used agents. This study showed that Escherichia coli is a most common causative agent of the urinary tract infection among haemodialysis patients, and Amikacin is the most susceptible antibiotic pattern.