Estimation of Fructosamine and HbA1c Levels in Type1 and Type 2 Diabetes Mellitus (Study in El-Obied City)

Abstract

This study was conducted to estimate serum fructosamine and HbA1c in patients with type 1 and type 2 DM. 100 blood samples (50 type 1, 50 type 2) were collected from patients during period from February to April 2016, chosen randomly from health insurance – diabetic center and El-obied teaching hospital, and 50 apparently healthy individuals as control group to evaluate and estimate serum fructosamine and HbA1c in diabetic patients. Nitroblue tetrazolium method used to estimate serum fructosamine concentration, and ion exchange chromatography method used for HbA1c estimation, and results were analyzed using statistical package for social science (SPSS) computer program. The study showed that the mean of fructosamine and HbA1c were significantly increased (P.Value 0.000)(P.Value 0.000) respectively for type 1DM. Mean ±SD for cases versus control: (8.19±4.31 versus 2.38± 0.4)mg/dl for fructosamine. (9.62±2.9 versus 4.69±0.64)% for HbA1c. Also the study showed that the mean of fructosamine and HbA1c were significantly increased (P.Value 0.000)(P.Value 0.000) respectively for type 2DM. Mean ±SD for cases versus control: (5.74±2.04 versus 2.38±0.4)mg/dl for fructosamine. (7.82±1.6 versus 4.69±0.64)% for HbA1c. The study also showed there was significant positive correlation between fructosamine and HbA1c for type 1 and type 2 DM. (P.Value 0.015, r = 0.341)( P.Value 0.001, r =457) respectively. Also the study showed there was a negative correlation between fructosamine and duration of type1 DM. (P.value 0.384, r = - 0.235). The study showed there was no correlation between fructosamine and duration of type 2 DM.(P.Value = 0.371, r= -0.138). It is concluded that the serum fructosamine and HbA1c were significantly increased in type 1 and type 2 DM, also singinficant correlation between fructosamine and HbA1c.