Cytomorphological Pattern of Peritoneal Fluid using Cytological Techniques

Abstract

This descriptive study was carried out in Khartoum North, Rebat university and Omdurman Military hospitals, during the period from February 2011 to March 2012 . The study was aimed to study the cytomorpholigical pattern of peritoneal fluid using cytological technique. .Seventy cytological smears were collected from patients with Ascetic their ages ranged between 10 to 85 years with mean age (45) years .Smears were prepared ,fixed and stained using Papanicolaou stain and Diff,s Quick stain then examined under the light microscope . The cellularity of smears were 35(50%)smears were cellular and 35(50%)smears were a cellular . Out of 35 cellular smears, cell pattern distributed as follow mesothelial cells 5 (14.2 % ), malignant cells were found in 4(11.4) smears, among which 2(5.7%) smears were females and 4 2(5.7%)smears were male. However most of the cellular smears showed dense inflammatory infiltrate, which were detected in 30(42.8%) of the study subjects. The infiltrate majority were of the inflammatory lymphocytes (57.1%), followed by cells constituting 20 neutrophils , finally plasma cells 2 (5.7%) . The study conclude that cytological changes were detected in ascetic fluid by cytological stains. Papanicolaou method is clearly superior and the most important for demonstration of the nuclear details, and particularly the presence of nuclear grooves, but achieving more accurate result requires use of both papanicolauou and diffs quick stains.