In Vitro Assessment of AntibacterialActivity of lactobacillus species Against Selected Bacteria Isolated from Sudanese Patients Suffering from Urinary Infection

Abstract

This was cross-sectional study had been carried out to isolate, identify and testing antimicrobial activity of UTI among 30 urinary tract infection patients attended, National Public Health laboratory during the period from April to May 2016 to determine antibacterial activity of lactobacillus ssp. against urinary tract infection causative bacteria isolate from Khartoum state. A total of 30 urine sample were collected from patient with urinary tract infection. These specimens were cultured onto Blood agar media and MacConkey's agar media incubate aerobically at 370C for 24 hours .The isolates were identified using conventional method, 100% sample showed significant bacterial growth. The percentage of these isolated uro-pathogen, E. coli was found to be the most frequently isolated pathogen 9 (30%) followed by K. pneumoniae8 (26.7%), Enterococcus faecalis6 (20%), Staphylococcus aureus5 (16.7%), Psudomonasaeruginosa1 (3.3%), Proteus mirabilis 1 (3.3%).When sensitivity testing was performed using stander disc diffusion method,Most of isolates was resistant to co-trimoxazole 24 (80%), norfloxacin 12 (40%), ciprofloxacin and nitrofurantoin were similar 10 (33.3%), Ninety (90%) specimens were sensitive to chloramphenicol and 10% isolated bacteria were resistant to it. For probiotics the pattern of sensitive was performed using ager well diffusion method showing following result Lactobacillus .acidipiscis 1 (3.3%) Lactobacillus rhamanosus 2 (6.9%), Lactobacillus.lactissub.sp. hodinae was more sensitive 4 (13.3%). probioticLactobacillus.lactissub.sp. hodinae capable of producing antimicrobial activity against urinary tract infection bacteria and higher antibiotic produce sensitivity chloramphenicol.