Role of Laminin -5 as Marker of Malignant Transformation in Colorectal Polyps

Abstract

This is a hospital based descriptive retrospective case study conducted in national public health laboratory during the period from July 2016 to October 2016, the study aimed to detect the role of laminin 5 in invasiveness in colorectal polyps. A total of 40 formalin fixed paraffin blocks previously diagnosed as colorectal polyps,14(35.0%) samples were juvenile polyps, 14(35.0%) samples were adenomas polyps and 12(30.0%) samples were hyper plastic polyps were selected for this study. One section of three microns was cut from each block and stained by immunohistochemical method (avidin biotin technique) for laminin 5 detection. The data obtained was analyzed using SPSS computer program version 20. The patient ages ranged between 3-80 years with mean age 43 years, most of patients 22(55.0%) were above 50 years and the remaining 18(45.0%) were less than 50 years. Regarding gender patient sex revealed that 11(27.5%) patients were female and 29(72.5%) patients were male. Positive expression of laminin-5 among study sampleswere 16/40 samples, distributed as follow inadenomas polyps7/14 samples, juvenile polyps 7/14 samplesand in hyper plastic polyps 2/12 samples, while negative expression of laminin-5 among study samples is 24/40 samples, the most negative expression of laminin-5 was seen in hyper plastic polyps account 10/12, adenomas polyps account 7/14 samples and juvenile polyps 5/14 samples with significant correlation between Laminin-5 and polyps types (P. value 0.047). The study revealed there is role of laminin -5 as marker for malignant transformation in colorectal polyps and laminin 5 expression is associated with adenomatous polyps and juvenile polyps; they are more common pre-cancerous lesions of colorectal cancer.