Detection of Extended Spectrum β-Lactamases among Escherichia coli Isolated from patients with Urinary Tract Infection in Al Noor Hospital, United Arab Emirates

Abstract

Extended-spectrum β-lactamases-producing Escherichia coli (ESBL-E. coli) are increasingly identified in health care facilities. The objective of this study was to detect ESBL among E. coli isolated from patients with urinary tract infection. Escherichia coli isolates were obtained from Alnoor hospitals. The isolates were purified by streaking on nutrient agar plates. Re-identification was done primarily by cultural characteristic, Gram stain and confirmed by standard biochemical tests using VITEK 2 system. Antibiotic susceptibility and β-lactamases detection were carried out also by VITEK 2 system. A total of 245 E. coli isolates (43 (17.6%) from males and 202 (82.4%) from females) were investigated. All isolates (100%) were susceptible to Impenem, Etrapenem and Meropenem, while (62%) were resistant to Amoxicillin and (60%) resistant to Ampicillin. Of the total isolates tested, 152(62%) were ESBL positive, and the rest 93(38%) were negative. This study showed high resistance of E. coli to antibiotics. Considerable number of E. coli were ESBL-producing. Regular monitoring and use of antibiotics should be encouraged. Further studies with large number of isolates and advanced techniques such as molecular methods are required to validate the results of this study.