Association of GSTM1Null Gene Polymorphism with Patients of Chronic Lymphocytic Leukemia in Khartoum

Abstract

Inter individual differences in susceptibility to hematologic malignancies may be mediated in part through polymorphic variability in the bioactivation and detoxification of carcinogens. The glutathione S–transferases (GSTs) have been implicated as susceptibility genes in this context for a number of Cancers. This is a Prospective case control study conducted in central research laboratory to study the association between GSTM1 null gene polymorphism and patients of chronic lymphocytic leukemia in Khartoum State in the period from February 2016 to January 2017. forty samples were collected from newly diagnosed patients of chronic lymphocytic leukemia attending to radiation and isotype center Khartoum _Sudan, and 30 samples were collected from normal individuals as control group, all samples were collected in (2.5) EDTA anticoagulant. then DNA was extracted by using salting out method.And polymorphism analysis for GSTM1 was determined using Allele specific polymerase chain reaction (PCR). The results were analyzed by statistical package for social sciences (SPSS) computer program. The results showed that (15%) of the cases have GSTM1 null genotype and (85%) of them have normal GSTM1genotype, (56.6%) of the control have GSTM1 null and (43.4%) have normal GSTM1 genotype. The association between GSTM1 null genotype and CLL is statically insignificant. The mean of hematological parameters (TWBCs count, Hb, MCV, MCH, MCHC, PCV, TRBCs count,blast percentage, lymphocyte percentage, neutrophil percentage, eosinophil percentage, monocyte percentage, platelet percentage ) in GSTM1 null cases was insignificant associated with CLL. Thestudy conducted that there are no association between GSTM1 null genotype and CLL.