Isolation and Identification of Uropathogens in Pregnant Women using CHROM agar

Abstract

Urinary tract infections (UTIs) are among the most common bacterial infections that lead patients to seek medical care. One hundred and fifty urine samples were collected from pregnant women at Families' Military Hospital during the period from April to May 2012. The samples were examined microscopically and chemically. Moreover all the samples were cultured on CHROM agar and cystine lactose electrolytes deficient (CLED) agar for primary isolation of the uropathogens. Thirty (20%) samples of urine were found to be positive for bacterial growth while the others 120 (80%); only seven of these were positive for the nitrite test and eight were positive for the leucocytes-esterase test. Seven (4.7%) samples gave positive bacterial cultures and all of these were also microscopically positive for bacteria. The isolated bacteria were 15 (34.1%) Escherchia coli, 11 (25%) Enterococcus faecalis, 6 (13.6%) Staphylococcus aureus, 4 (9.1%) yeast, 3 (6.8%) Staphylococcus saprophyticus, 2 (4.5%) Pseudomonas diversus, 1 (2.3%) aeruginosa, Citrobacter 1 (2.3%) freundii and Citrobacter 1 (2.3%) Klebsiella pneumoniae. CHROM agar enabled us to isolate 43 (97.7%) uropathogens while the CLED agar isolates just 37 (84.1%) isolates.