Molecular Characterization and Sensitivity Patterns of Pseudomonas aeruginosa among Patients with Different Diseases

Abstract

This study was designed to investigate the presence of Pseudomonas aeruginosa among patients with different diseases and to subject the isolated P. aeruginosa to molecular characterization and antibiotic susceptibility patterns in Khartoum state. The study was performed during the period from February to June 2011. It included 150 samples (different types of bacteria were isolated including P. aeruginosa 74 (49.3%) and others 76 (50.7%)) from urine, wounds, eyes, ears, vaginal infections, sputum, shunts, pleural fluid aspirates and cerepro spinal fluid and asitic fluid. This samples were collected and examined in Khartoum Teaching Hospital and the research laboratory at the Sudan University of Science and Technology (SUST). These were collected from patients with different sexes and ages. The samples were cultured on cystine lactose electrolyte deficient agar (CLED), MacConkey agar, blood and chocolate agar, incubated aerobically at 37oC. Gram stain was done for bacterial isolates. Biochemical tests were used to identify isolated organism (bacteria). Susceptibility tests were designed to determine the antibiotic resistance of isolated P. aeruginosa. Molecular characterization (polymerase chain reaction "PCR") amplification target genes IMP-7 and IMP-10 were done for isolated P. aeruginosa. The study revealed that P. aeruginosa was isolated from samples was (49.3%) and others (50.7%) were resistant to (20) carbenicillin (CB) percentage was (13.3%). The antibiotic resistance tests showed that (74) of P. aeruginosa isolates (49.3%) were resistant to carbenicillin. DNA extraction by Boiling-centrifugation method from culture were done for (57) isolates, PCR showed (2) isolates (26) (45.6%) were IMP-7 primer reorganized and (31) isolates (54.4) were not IMP-7 primer, also PCR showed (2) isolates (31) (54.4) were IMP-10 primer recognized and (26) (45.6%) were not IMP-10 primer. This study showed that the identification of P. aeruginosa by molecular characterization (PCR) and sensitivity patterns among patients with different diseases in Khartoum state. Amplification of IMP-10 gene was more active than IMP-7 gene which is desirable due to the unlimited of this genes in the strains of P. aeruginosa. ‫