Comparison of Helicobacter pylori Immunoglobulin G Antibody Detection with Helicobacter pylori Antigen Detection in Patients with Gastritis

Abstract

Infection with Helicobacter pylori has been associated with the pathogenesis of chronic active gastritis, gastric and duodenal ulcer disease. Detection of immunoglobulin G antibodies to H. pylori by an immunochromatographic test (ICT) offers a simple alternative to direct detection of the organism in biopsied tissue by culture or histopathological methods. The aim of this study was to compare the rapid flow-through membrane-based enzyme immunoassay for the detection of human immunoglobulin G antibodies to H. pylori with H. pylori antigen detection in human stool. Clinical evaluations were performed with 150 patient serum and stool samples obtained from Yastabshiroon Hospital. Stool samples were obtained by collection in clean dry container at the same time as the serum samples; were serologically categorized for the presence or absence of H. pylori antigen. sensitivity and specificity for this rapid enzyme immunoassay were 98% and 91%, respectively, compared directly with antigen detection results. These results immunochromatographic indicate test (ICT) that for this rapid detection of immunoglobulin G antibodies is a useful technique to determine H. pylori infection status and is a viable alternative to invasive endoscopic procedures.