Assessment of Plasma Alpha Amylase Level in Sudanese with Type 2 Diabetes Mellitus

Abstract

Diabetes is known caused by relative or absolute insufficiency of insulin secretion and/or concomitant resistance to the metabolic action of insulin on target tissues, then hyperglycemias developed. Alpha amylases are enzymes which hydrolyze starch molecules to give diverse products including dextrins and progressively smaller polymers composed of glucose units which cause increase blood glucose. Therefore across sectional study conduct in Abdoon Seed Ahmed Polyclinic Centre in Khartoum during the period from April to June 2012 to assess plasma alpha amylase activity in Sudanese type 2 diabetic patients.5 milliliters of venous blood was collected from each of 40 females (mean age 40 years) and 30 males(mean age 51 years). Blood was separated into lithium heparin container (3mls) for measurement of alpha amylase, glucose and renal function tests, and EDTA containers for measurement of HbA1C.Twenty fife apparently healthy volunteers were included as control 14 males (mean age 51 years) and 11 females (mean age 40 years). Alpha amylase activity was estimeted by using direct substrate (CNP- G3).HbA1C was estimated by using Full automated immunoflouresnt technique, and glucose was estimated by glucooxidase enzymatic method. Data were analyzed by SPSS computer program. There was insignificant increase in alpha amylase activity of study group compared to control group (P value > 0.05) and there was significant deference in study groups and control groups in fasting plasma glucose and HbA1C (P value <0.05). There was no significant difference between diabetic males and diabetic females regarding alpha amylase, FBG, and HbA1c (P value > 0.05). It was found that there is positive correlation of α amylase with the duration of disease and age.