Evaluation and Comparison of Minimum Inhibitory Concentration Of Various Antibiotics on Common Organisms before and After Cold Shock

Abstract

This study was carried out to study the effect of cold shock on certain organisms which routinely isolated from a variety of clinical specimens, Escherichia coli, Enterococcus faecalis,and Salmonella typhi on their growth and evaluation and compression of minimum inhibitory concentration of these organisms with amoxicillin, cephalexin, ceftriaxone, ciprofloxacin, chloramphinicol and co- trimoxazole antibiotics before and after cold shock in -20c for at 1 hour. This study revealed that clinical assessment showed that Eschericia coli before cold shock at -6 dilution gave a result 1.8 x 10 8CFU/ml, at -7 dilution gave 0.5 x109CFU/ml, at -8 dilution gave 0.4 x1010 CFU/ml, and after cold shock the result was 0.8 x108 in -6 dilution and at -7 dilution gave 0.3 x109CFU\ml and at -8 is 0.2x1010 CFU\ml. The result for Salmonella typhi before cold shock in -6, -7, -8, and -9 gave uncounted colonies, and at -10 gave 4.1 x10 12 CFU\ml, and after cold shock, at -6 dilution produced 4.4 x108, at -7 dilution produced 3.1 x109CFU/ml, and at -8 dilution produce 1.6 x1010CFU/ml, and at -9 and -10 dilution gave no growth. Enterococcus faecalisbefore cold shock in -6 tube dilution and -7 dilution the result is uncounted colonies, at -8 dilution it gave 2.05 x10 11CFU/ml, at -9 produce 1.68 x1012and at -10 7.8 x1012CFU/ml. After cold shock at -6 uncounted, at -7 gave 1.63 x1010, at -8 gave 1.33 x1011CFU/ml and at -9 dilution gave 1.02 x1012 and -10 gave 3.9 x1012 Result of minimum inhibitory concentration for Escherichia coli before cold shock is resistant to amoxicillin, cephalexine and co-trimoxazole, the result with ceftriaxone is 240 mcg, Ciprofloxacin 7.5 mcg, chloramphinicol 1 mcg. After cold shock Amoxicillin, cephalexin, ceftriaxone are resistant, ciprofloxacin 3mcg, chloramphinicol 1mcg. For Salmonella typhi before shock Amoxicillin, co- trimoxazole and ciprofloxacin are resistant, cefalexine 30mcg, ceftriaxone 0.008 mcg, chloramphinicol 0.1 mcg, and co-trimoxazole 0.1 mcg. After cold shock amoxicillin, cephalexin and ciprofloxacin are resistant, ceftriaxone 0.008mcg, chloramphinicol 0.1 mcg and co-trimoxazole 0.05 mcg. For Enterococcus faecalis before cold shock with amoxicillin 4 mcg, cefalexine 3 mcg, ceftriaxone 0.01 mcg, ciprofloxacin 10 mcg, chloramphinicol 0.5 mcg, and co-trimoxazole 0.1 mcg. After cold shock amoxicillin 4 mcg, cephalexin 3 mcg, ceftriaxone 0.01 mcg, ciprofloxacin 10 mcg, chloramphinicol 0.5 mcg and co-trimoxazole 0.1 mcg.