Design of detector for vaccines container based on gold nanoparticles

Abstract

Vaccines container detector based on gold nanoparticles was synthesized according to plant seeds extract method which is simple, efficient, economic and environmentally In this study two types of seeds were used to produce gold nanoparticles: black seed (Nigella sativa) and fenugreek seed (Trigonella foenum graecum) extracts as reducing agent to reduction of gold precursor of Au+3(HAuCl ) to Au0 in the presence of a stabilizer (Gum Arabic) which keeps nanoparticles apart, thus avoiding their aggregation. The produced gold nanoparticles were characterized by using and ultraviolet-visible absorption spectroscopy, X-ray diffraction and transmission electron microscopy. It was found that the concentration of the precursors affects the size of the nanoparticles. Typical images obtained for black seed colloids consist of almost uniformly sized spherical nanoparticles, while fenugreek consist of different shapes of gold nanoparticles. The generated gold nanoparticles were used as detector from which can change its color irreversibly when the solution become frozen (0 0C ± 0.5) by adding chemical material (glucose and silica gel) to give faster respond to temperature change. For the detector find that the more increase of the amount of glucose added to the gold nanoparticles solution whenever given the change in the color characteristics and faster. As well as the selection of glucose gives a change in the characteristics and best results when compared to choose silica gel. A find that the fenugreek in response to the change in temperature faster than black seed as well as change color characteristics in a short time, which gives a good indication to an application in vaccines to check the degree of preservation and freezing.