Determination of Antibiogram of Pseudomonas aeruginosa Isolated from Renal Failure Patients on Haemodialysis in Khartoum State

Abstract

Pseudomonas aeruginosa (P. aeruginosa) infection in renal failure patients undergoing haemodialysis constituted a true health problem. This study was essentially designed to determine antibiogram of P. aeruginosa isolated from haemodialysis patients. The study was carried out in the period from February to May, 2015. The isolates P. aeruginosa were obtained from the Research Laboratory, College of Medical Laboratory Science, Sudan University of Science and Technology (SUST). The isolates were checked for purity by streaking on nutrient agar and examined microscopically. Re-identification was done by conventional microbiological methods, including colonial morphology, Gram’s stain and biochemical tests. Antibiogram of the isolates was done by disk diffusion method, the antibiotics used were Ciprofloxacin, Imipinem, Gentamicin, Amikacin and Ceftriaxon. Re-identification of the isolates confirmed that all isolates (n=12) were P. aeruginosa. Study on antibiogram of these isolates revealed 100% susceptibility to Ciprofloxacin and Imipenem, 83.3% to Gentamicin, 75% to Amikacin and 58.3% to Ceftriaxone. The study concluded that P. aeruginosa in haemodialysis patients can be treated successfully using antipseudomonal drugs. Regular check up of antibiogram among large number of clinical isolates of P. aeruginosa recovered from hemodialysis patients is highly recommended