Determination of Prothrombin time and International Normalized Ratio by Using Manual and Semi automated Methods in Khartoum Teaching Hospital Laboratory

Abstract

This is descriptivecross sectionalstudy carried out in the Khartoum teaching hospital research and laboratory unit to determine the Prothrombin time and International normalized ratio. The Prothrombin time and International normalized ratio was measured in100 blood samples.4.5ml of venous blood was drown from each patients placed in tri sodium citrate containers then centrifugated to get platelet poor plasma. PT measured by the manual method and semi automated method (DiagnosticaStago). The aim of this study to compare the reliability of prothrombin test using manual and semi-automated method coagulation analyzer. Results were analyzed by Statistical package for social science average ±SD and P.value was obtained. It has been found that there was no significant variation between PT and INR values when using the manual and semi automated with P.value (≥0.05). The result for the prothrombin time test obtained by semi automated is reliable and is statistically equivilant to those obtained by the manual method. The study concluded that there weretwo methods gave the same results indicating good calibrated machine.