Development and Validation of Spectrophotometric Method for Determination of Penicillamine (PA) in Pharmaceutical Formulation Using 4-Chloro-7-Nitrobenzo-2-Oxa-1, 3-Diazol (NBD-Cl)

Abstract

A sensitive and simple spectrophotometric method has been proposed for the determination of D-Penicillamine (PA) in pharmaceutical formulation. The proposed method is based on the reaction between the PA and 4-chloro-7-nitrobenzo-2-oxa-1, 3- diazole (NBD-Cl) at alkaline medium (pH=10.5) to form deep brown-purple adduct, exhibiting maximum absorption ( max  ) at 465nm. Under optimized reaction condition, the method was linear in the concentration rage 1-15μgmL 1 . The method was applied successfully to the determination if PA in pharmaceutical dosage form. The results were in a good agreement with the official USP method. The method is useful for routine analysis of PA in quality control laboratories.