Stability Study of Atenolol and validation of its HPLC Quantification method

Abstract

In this study the stability of Atenolol in solid and liquid forms was investigated, towards high temperature, sunlight, UV light, some common excipients and hydrolysis in acidic media and alkaline media, using high performance liquid chromatography ( HPLC ).the results obtained were compared with the UV spectrophotometric method. A new stability indicating ( HPLC) method was validated for assaying Atenolol. It was found that Atenolol was thermally stable in solid and aqueous solution form up to70oC. On exposure to UV radiation, however, the solution form was unstable, and the photo scanning of Atenolol solution with different pH values decomposition showed first order kinetic reaction. UV showed bathochromic (red) shift as ƛ max was changed to longer wavelength with increased pH of the solution. Atenolol hydrolysis in acidic and alkaline media showed also first order reaction kinetic and the reaction rate was temperature dependant. Both hydrolyzed compounds from acidic and alkaline media have same retention time in the chromatograms. Comparing the results of hydrolysis obtained from ( HPLC ) with that obtained by UV. Spectrophotometric analysis, the UV results showed no hydrolysis .The method is not stability indicating method. A new analytical high performance liquid chromatography ( HPLC ) method was validated for Quantification assay for Atenolol with IVaccuracy 99.55% and linearity 99.99% and compared with (USP) method.