Comparison of serum alpha amylase level between type one and type two diabetes mellitus among Sudanese in Khartoum state in 2015

Abstract

Diabetes Mellitus result from insufficiency of insulin secretion or concomitant resistance to the metabolic action of insulin on target tissue, then hyperglycemia as developed. Alpha amylase is enzyme which hydrolyses starch molecules to give smaller polymers composed of glucose unit which cause increase blood glucose. Therefore analytical, case control and hospital base study conduct in Ahmed Qasum hospital in Bahri in Khartoum state (Sudan) during the period from March to Jun 2015 to compare serum alpha amylase level in diabetic mellitus type one and type two . Thirty-three diabetic patients in type one and thirty-three diabetic patients in type two were selected from Ahmed Qasum hospital as test groups, thirty-three healthy person as control groups. Three millimeter of venous blood was collected from each person; blood was separated into plain container for measurement of Alpha amylase. Alpha amylase activity was estimated by using commercial reagent Kitts from Bio System Company, statistical package for social science (SPSS version 16) computer software was used for data analysis. There was significant decrease alpha amylase activity in diabetes mellitus type one compared to type two (P value 0.034) and control (P value 0.001). It was found that there is negative correlation of alpha amylase with duration of disease and age among type one Diabetes mellitus. Also show insignificant decrease alpha amylase activity in diabetes type two compared to control (P value 0.150) It was found not correlation of alpha amylase with duration of disease and age among type two Diabetes mellitus. From this study it is concluded that; alpha amylase activity in diabetes type one decrease than type two. Due to diabetes type one has insulin insufficient more than type two that effect on AMY.