Measurement of CompleAte Blood Cell Count of Sudanese Pregnant Women at Third Trimester - Khartoum Locality

Abstract

This is a case control study, conducted in Khartoum locality during the period from February to March 2015, to determine CBC (Hb, RBCs, HCT, MCV, MCH, MCHC, Platelets, WBCs, Leukocyte differential count, RDW, and MPV) of Eighty (80) healthy Sudanese pregnant women at third trimester as case and forty (40) non pregnant women at similar age in Khartoum locality were used as control in this study. pregnant women at third trimester were informed about the study and agreed for participation as case. A questionnaire was designed to collect information about the study group such as age, number of pregnancies, month of pregnancy, history of abortion and whether they visit the clinics regularly. 2.5 ml venous blood was collected in EDTA anticoagulant container. Automated hematological analyzer (Sysmex KX – 21N) was used to measure Complete Blood Count, and the results were analyzed by independent T test and one way ANOVA test of the SPSS computer programme. The means were found as following : Hb (11.8±1.19 g/dl), Hct (35.2±3.0 %), TRBCs ( 4.1±0.35 ×1012/L), MCV ( 84.1±5.62 fl ), MCH (27.8±3.73 pg),MCHC ( 33.4±1.48 %), RDWSD (44.3±3.90 fl), RDWCV(14.2±1.31%), TWBCs (7.4±2.44×109/L ), lymphocytes percentage ( 26.6±9.90 % ), neutrophils percentage ( 63.4±11.31% ), lymphocytes absolute (1.8±0.66×109/L), neutrophils absolute (4.8±2.19×109/L), platelets count ( 212± 64.5×109/L), PDW (14.9±2.19), MPV (9.5±1.60 fl) in case and Hb(12.0±1.19 g/dl), Hct (38.7±3.55 %), TRBCs ( 4.5±0.45×1012/L), MCV ( 85.2±8.07 fl ), MCH (26.6±2.65 pg),MCHC ( 31±1.33 %), RDWSD (46.6±6.13fl), RDWCV( 14.3±1.29%), TWBCs ( 5.9±1.63×109/L ), lymphocytes percentage ( 38.9 ±8.01%), neutrophils percentage ( 49.1±11.89% ), lymphocytes absolute (2.2±0.46×109/L), neutrophils absolute(3.0±1.41×109/L), platelets count ( 289.5±79.74×109/L), PDW (15.2±0.82), MPV (9.3±1.21 fl) were found in control group. This study was concluded that: there was significant decreased (p≤ 0.01)in means of Hct, TRBCs, RDWSD, lymphocytes percentage and absolute count and platelets count in pregnant women when compared with control, significant increased (p=0.00) in means of MCHC, TWBCs and neutrophils percentage and absolute of pregnant women more than control.There was significant increased in means of MPV (p=0.01) of with abortion when compared to without abortion. According to numbers of children there was significant increased (p=0.01) in means of Hct, MCV, and RDWSD in < 2 more than > 2 children. According to their age group there was significant increased (p=≤ 0.03) in means of Hct, MCV, and MCH in < 30 more than > 30 years.