Detection of CDX2 Tumor Marker and Human Papilloma Virus (HPV) in Esophageal Tumor among Sudanese patients using Immunohistochemistery and Polymerase Chain Reaction

Abstract

This is a descriptive retrospective study conducted at Ibn Siena hospital and Sudan University of Science and Technology during the period from April 2014 to October 2014.The study aimed to detecte CDX2 tumor marker and human papilloma virus (HPV) in esophageal tumor among Sudanese patient using immunohistochemical method and Polymerase chain reaction. In this study 30 samples were collected from patients previously diagnosed with esophageal tumor (20of them with esophageal cancer and 10 of them with benign tumor). The p a t i e n t ' s age ranging between 8 to 82 years with mean age 37 years, with no association between age group and type of esophageal tumor. From each block two sections were cut, one for CDX2 detection and other for HPV detection , also 20 micro liter was cut from each block in ependorff tube for detection of HPV type 31 gene using PCR. SPSS version 16 computer programm was used to analyze the data, mean, frequency and chi square were calculated. Out of thirty patients twenty (66.7%) were males and ten (33.3%) were females, with the ratio 2:1. CDX2 was positive in four samples (13.3%) all of them were malignant, and negative in 26 (86.7%) . Seven samples (23.3%) were positive for humanpapilloma virus and 23 (76.7) samples were negative by using immunochistochemistery. The detection of human papilloma virus type 31 was done for 30 samples by using PCR two samples (6.7%) were positive, and 28 (93.3%) were negatives. The study concluded that, there is no association between CDX2 expression and HPV infection with the type of esophageal tumor.