https://repository.sustech.edu/handle/123456789/1312 2026-04-06T05:56:02Z 2026-04-06T05:56:02Z Mohammedali, Mohammed Abass Albashire Supervisor, -Siham Elias Suliman Mohammed Co- Supervisor, -Mohamed Abdelsalam Abdella https://repository.sustech.edu/handle/123456789/28141 2023-02-22T10:53:45Z 2022-02-22T00:00:00Z

Title: Investigation of Some Bacterial Zoonotic Diseases of Fish in Khartoum State-Sudan Authors: Mohammedali, Mohammed Abass Albashire; Supervisor, -Siham Elias Suliman Mohammed; Co- Supervisor, -Mohamed Abdelsalam Abdella Abstract: Freshwater fish play a major role in feeding of people near to Nile River specially in Sudan.This study was conducted to investigate some bacterial zoonotic diseases of fish from November 2019 to December 2020 in Khartoum state.A total of 50 fish were examined at Almawrada fish market, Omdurman locality, Khartoum state. One hundred and fifty tissue samples (gills,livers and kidneys) were taken from 50 different breeds of fish were 19 Nile tilapia(Oreochromis niloticus),18 Synodontis (Synodontis schall), 3 Nile puffer(Tetraodon lineatus),4 Bagrus catfishes (Bagrus bayad) and 6 Nile Perch(Lates niloticus). Fish postmortem,bacteriological diagnosis and conventional PCR were applied for confirmation.The common lesions observed externally were darkness of the skin, hemorrhage in the fins base, eyes and different parts of the body, abdominal distention, congested gills and increasing in mucous secretion. The postmortem findings showed white serous fluid in the abdominal cavity and sometimes tinged with blood and pale or congested livers and kidney. Three swab samples obtained from each fish from gills, livers and kidneys.The bacteriological isolation and identification samples revealed that the prevalence of bacterial pathogens among naturally infected freshwater fishes were Streptococcus iniae (21;14%),Staphylococcus aureus(47;31.3%), Salmonella spp(44;29.3%), Escherichia coli(44; 29.3%) and Pseudomonas spp(17; 11.3%).The results of risk factors shown that there was age of fish more associated with E.coli infection.When PCR was used obvious clear single band was appeared for E.coli.In conclusion the bacteria identified in this study are considered as zoonotic bacteria of significant economic important .Fish contamination from the surrounding of young fish are more susceptible to infection by E.coli and salmonella species.PCR is rapid and realistic tool for diagnosis of fish infection. Description: Thesis

2022-02-22T00:00:00Z Jebril, Ahmed Abdelraheim Abdalla Supervisor, -Mohammed Abdelsalamabdalla https://repository.sustech.edu/handle/123456789/28139 2023-02-22T10:38:59Z 2021-08-21T00:00:00Z

Title: Epidemiology Of Brucelosis In Camels In West Kurdufan State - Sudan Authors: Jebril, Ahmed Abdelraheim Abdalla; Supervisor, -Mohammed Abdelsalamabdalla Abstract: A cross-sectional study was carried out fromSeptember 2018 to April 2019.The aim of this projectwas to study Brucellosis in the camels (Camelus dromedarius) to estimation the prevalence of brucellosis in thecamels and investigate risk factors associated with the disease in West Kurdufan state. Atotal of 500 serum and whole blood samples were collected from camel in three selected localities in West Kurdufan State, the total of camels examined in this study 49% (n=245) from Alnehood, 42.4% (n=212)from Ghubaish, and 8.6% (n=43) from Elkhwai Localities. From the total camels tested 28.8% (n=144) were males, while 71.2 %( n=356) were females respectively. All samples were screened by the RBPT, and 3.8%(19/500) were positive by the test. All samples were further tested by the serum agglutination test (SAT) test, and of the 19 RBPT positive samples,17 were found positive by the SAT test. Among the sixty herds included in the study 15were found positive (25%) .The investigated individual risk factors included: localities, sex, age groups, sexes while herd sizes, management types, having aborted animals in the herd, sharing males between herds for breeding purpose, contact with other ruminants ,contact with other camel herds at pasture and water points and awereness of herdman about brucellosis were the management risks.The study revealed that 3.8% of samples tested and 25% herds were seroposetive for brucellosis with increase in seroprevalence of disease in Elkhwai 16.3% ,Alnehood 3.7% and Ghubaish 1.4%.(OD Ratio =13.546 , CI = 3.347-54.822 , P-value =0.000).The result showed that the prevalence is relatively higher in females 3.9% than males3.5%. The seroprevalence of brucellosis according to herd size were2.8%,3.3% and 4% in small, medium and large herd sizes, respectively.This increase in seropositivity is not significant statistically(p.value=0.920).The study revealed that the camel contacts with other camel herds in pasture and water points were higher prevalence (9.2%) than herds not in contact(2.2%) (OD Ratio =0.119, CI = 0.039-0.363, P-value =0.000), whereas camels reared with ruminants (sheep, goat and cattle) showed prevalence (6.7%) higher than that kept alone (2.2%). (OD Ratio =0.307, CI = 0.119-0.796, P-value =0.015). This study showed the status of seroprevalence and with exception of (localities, contact with other herds and species) the statistical analysis by using chi square didn't show any effect of other risk factor on prevalence of brucellosis among camels in West Kurdufan State. Out of the 500 whole blood sample tested by polymerase chain reaction (PCR) none of the sample was found positive. Description: Thesis

2021-08-21T00:00:00Z Abu Zaid, Hanan Abd Eljalil Supervisor, -Mohamed Abdelsalam Abdella https://repository.sustech.edu/handle/123456789/27968 2022-12-26T11:53:35Z 2022-06-22T00:00:00Z

Title: Bacterial Contamination of Cow Raw Milk in Khartoum State, Sudan. Authors: Abu Zaid, Hanan Abd Eljalil; Supervisor, -Mohamed Abdelsalam Abdella Abstract: This study was conducted to isolate and identify some pathogenic bacteria which contaminate raw milk and to assess the risk factors associated with milk consumption in the seven localities of Khartoum state (Khartoum, Jabal Awlia, Bahri, Sharg Elnil, Omdurman, Ombadda, Karary) and to estimate the knowledge and awareness of dairy workers and milk consumers about milk borne diseases by aquestionnaire which conducted for 200 milk workers and consumers fromJuly 2018 to July 2020and Atotal of 102 samples of raw cow milk were collected in winter , summer and autumn seasons from December 2018 to October 2019 from milk equipment , udders of milking cows, and venders, samples were analyzed and examined for detection of coliform bacteria and some pathogens contaminate raw milk causing milk borne diseases and effect on public health .The isolates were identified morphologically and biochemically and the results revealed that all examined samples showed growth of Gram negative bacteria (233 isolates) and recorded as follow : E.coli spp. 75(32.19%), Klebsiella spp. 30(12.88%) Salmonella spp.15(6.44%), Shigella spp. 20(8.58%), Proteus spp 66(28.33%),and Pseudomonas spp .7(11.58%) . According to the source of collection the isolates were 65(27.9%) from the udders , 93(40%) from equipment , and 75(32.1%) from vendors and this indicate high contamination in milk equipment , and according to the seasons the isolates were 35(15.0%) in winter, 66(28.3%) in summer , and 132(56.7%) in autumn respectively ,and this indicates high contamination in autumn season A questionnaire survey was conducted to estimate the knowledge and awareness of dairy workers and milk consumers about milk borne diseases associated with milk contamination and effect on public health, the survey was done from July 2018 to July 2020 in Khartoum state. A total of 200 respondents were selected randomly(30 dairy holders, 50 farm workers, 25 milk sellers, and 95 milk consumers) using a structural questionnaire with questions consisting demographic characters of the respondents from the seven localities of Khartoum state (Khartoum, Jabal Awlia, Sharg Elnil, Bahri, Omdurman, Ombadda, Karary). The results revealed that the age 18-45 years in dairy holders were 13(43.3%), milk farmers 40(80.0%), milk sellers 17(68.0%), and milk consumers were 20(21.0). Most of the respondents were studied primary education .About 143(79.17%) of them were known milk borne diseases,but 57(20.83%)were not known the disease. The incidence of the infection by consuming raw milk was 16.7% in dairy holders, 46.0% in milk farmers, 8.0% in milk sellers, and 30.5%in consumers. From the results, the conclusion was that contamination of milk by bacteria is high and because of this dairy workers need more awareness about sanitary measures. Description: Thesis

2022-06-22T00:00:00Z Babikir, Intisar Mohamed Osman Supervisor, -Galal Eldin Elazhari Mohammed Co-Supervisor, -Enaam Mohamed ElSanousi https://repository.sustech.edu/handle/123456789/27958 2022-12-22T11:03:50Z 2022-10-15T00:00:00Z

Title: Evaluation of Different Serological tests for diagnosis of Brucellosis in Camels and Male Goats Intended export in Sudan Authors: Babikir, Intisar Mohamed Osman; Supervisor, -Galal Eldin Elazhari Mohammed; Co-Supervisor, -Enaam Mohamed ElSanousi Abstract: A total of 5700 samples of blood were collected from animals intended for export with a healthy appearance, with two thousand samples (2000) from male camels, seven hundred samples (700) from female camels, and three thousand samples (3000) of male goats. All animals are not vaccinated. In this research, four tests were performed on blood serum, namely examination of samples for the presence of antibodies to Brucella with a rapid test, which is the RosBengal RBPT test. The positive samples were examined by two confirmatory tests, namely the SAT and the BAPA test, and finally by the cELISA-bound enzyme competitive immunoassay. The results were as follows: The RBPT examination excluded 21% of male camels, 26% of female camels, and 14% of male goats. It is a very large percentage compared to other tests. The SAT test was less remote for positive animals, where the percentage of deported males was 12% and females 14.5%, and goats were 3.5%. As for the BAPA examination, it excluded 13% of male camels, 14.5% of females, and 3.5% of goats. As for the cELISA test, it confirmed the positive rate of 7.4% of male camels, 7.4% of female camels, and 1.5% of goats. Statistical analyses were carried out using the Statistical Package for Social Sciences version SPSS prevalence and cross-tabbing were computed. Associations in the Chi-square test and logistic regression model were deemed significant when p≤0.05. All tests consider to be significant because the P value ranged from .000 to .003 and all the results less than .05. Description: Thesis

2022-10-15T00:00:00Z