Abstract:
This study was carried out in Khartoum state during the
period October
2008_March 2009 to determine frequency of
sonnei in patients
Shigella
with bacillary dysentery and its antimicrobial resistance.
A total of 253 stool specimens were collected from
patients to have
bacillary attended Haj Elsafi hospital, Omdurman Teaching
Hospital and
Alban jaded hospital . The specimens were cultured in
selnite F broth
for overnight, and then subculture on
deoxycholate to
Xylose lysine
isolate the causative agents. The identification of the
isolated bacteria
bacteria was done by colonial morphology, Gram stain
and API 20 E
system.
Antimicrobial resistance of each isolate was
determined by modified
Kirby – Bauer disk diffusion method. E . test was used
to determine
minimum inhibitory concentration of antimicrobial agents.
The result reveled that shigella sonnei was isolated
from 5 specimens.
Study on antimicrobial susceptibility test reveled that the
resistance rate of
Shigella sonnei was 100 % to ceftazidime and
tetracycline, 0 % to
ciprofloxacin, gentamicin and choloramphenicol.
The minimum inhibitory concentration ( MIC ) of were
0.001μg to
choloramphenicol, 0.5μg to tetracycline, 0.10 μg to
gentamicin, 0.001μg
to ciprofloxacin 0.01 μg to ceftazidime.
The MIC50 and MIC90 of antibiotics were, 0.5μg each to
tetracycline,
1.1
μg
ciprofloxacin, 0.10 μg gentamicin, 0.001 μg
choloraphenicol, and
the MIC50 of ceftazidime was 0.01 μg and the MIC90 of
ceftazidime was
0.1 μg.
The study concluded
sonnei is slighty high.
that
the
presence
of
Shigella
The antimicrobial resistance for Shigella sonnei was high too.
