Abstract:
This study was carried out in Khartoum State. Patients with community-acquired
urinary tract infection attended to Ibrahim Malik and Khartoum Teaching Hospitals
during the period February to April 2009.
A total of 103 urine specimens were collected. They were cultured on Blood and
MacConkey's agar's for primary isolation of pathogen, identification of the pathogens
were done by colonial morphology, gram stain and biochemical testes using API 20 E
.Modified Kirby-Bauer disc diffusion method was adopted to determine the resistance
rate of pathogens above against (nitrofuratoin, amoxycillin, nalidixic acid, co-
trimoxazole and amoxyclav). Minimum Inhibitory Concentration MIC of antibiotic
above was determining by E-test. Sixty five (65) (63.11%) specimens were found
growth and (38) (36.89%) were not growth. E. coli was show to be the most common
bacteria isolated (39) (60%) followed by K. pneumonia (11) (16.93%) followed by S.
aureus (7) (10.76%) followed by P. mirabilis (6) (9.23%) and followed by Ps.
aeruginosa (2) (3.07%).
Antimicrobial susceptibility analysis revealed that resistance rate of E. coli was
(66.6%) to amoxycillin which is highly resistance. The MIC of E. coli lowest in
co-trimoxazole (0.1-1μg/ml). The MIC 50 and MIC of antibiotic with E. coli were
also lowest in co-trimoxazol were (0.5 μg/ml) (1 μg/ml) respectively.
Study on antimicrobial susceptibility test revealed that the highly resistance rate of
K. Pneumonia was (100 %) to amoxycillin. The low MIC was amoxyclav
(0.01 - >240 μg/ml).The MIC50 and MIC90 were low to amoxyclav and amoxicillin (4
μg/ml) (<240μg/ml), resistance rate of S. aureus was highly (85.7%) in
amoxyclav.MIC of co-trimoxazole was (0.1 μg/ml) lowest one. MIC50 and MIC90
were also low in co-trimoxazole (0.1μg/ml) in both, P. mirabilis resistance rate was
(100%) to amoxyclav which is highly resistance. MIC of amoxyclav was (60-120
μg/ml).The MIC50 and MIC90 were (0.5 μg /ml) to co-trimoxazol. While in Ps.
aeruginosa were (100%) to nitrofuration, amoxycillin, co-trimoxazole and
amoxyclav. MIC of nalidixic acid and co- trimoxazol were (60 - <240μg/ml). The
MIC50 and MIC90 of were (<240mg/ml) in Nitrofuration, amoxycillin, nalidixic acid,
co- trimoxazol and amoxyclav.
It is concluded that the uro -pathogens isolated during this study, were capable to
resist all antimicrobial agents traditionally used in treatment of UTIs.
