Abstract:
This study was carried out in Khartoum state during the period from November
2008 to April 2009, to isolate and identify uncommon enterobacterial species from
clinical specimens.
Three
hundred
and
eleven
urine
specimens, one hundred and twelve
wound
specimens, and three hundred and eight stool specimens were collected from
patients attended Khartoum Teaching hospital, Gaffer Iben Auff specialize Hospital
for children, and Omdurman Teaching Hospital. The urine and wound swabs were
cultured on blood agar and MacConkey's agar for primary isolation of pathogens.
Stool specimens were cultured in selenite F broth and incubated. Then subcultured
on xylose lysine deoxycholate agar. Identification of the isolates was done by
,
colonial morphology, Gram s stain and biochemical tests using
API
20 E.
Modified Kirby-Bauer disc diffusion method was adopted to determine the
resistance of
the isolates
to
some
antimicrobial
agents. Minimum inhibitory
Concentration (MIC) of chloramphenicol, ciprofloxacin, tetracycline, gentamicin,
ceftazidime, amikacin, ceftriaxone, tecarcyline,
amoxicillin,
amoxyclav,
nalidixic
nitrofuratoin,
acid, cephotaxime,
determined by E. test.
and
co-trimoxazole,
tobramycin were
Out of 731 specimens investigated, 5 uncommon enterobactrical species were recovered.
These isolates were Salmonella choler.arizonae, chroactrum anthropi, Enterobacter
intermedius, Citrobacter youngae, and Pasturella multocida .
The majority of the isolate were resistant to antimicrobical agents.
The study concluded that uncommon enterobactrical species exist in clinical specimens.
