Abstract:
This study was carried out in Khartoum State during the period from
November 2008 to March 2009, to determine the frequency and antimicrobial
resistance of P. vulgaris in diabetic patients with
septic wounds.
One hundred and twelve wound swabs were collected from patients attended
Mac Nimir Complex for Diabetic and Surgery, Bashair hospital, and Jabir Abuizz
Diabetic Centre. The specimens were cultured on blood
and MacConkey's agars
for primary isolation of the pathogen. Identification of the isolates was done
by colonial morphology, gram’s stain and biochemical tests using API 20 E .
Out of the one hundred and twelve wound swabs examined, 6 (5.4 %) P.
vulgaris were recovered.
Modified Kirby-Bauer disc diffusion method was adopted to determine the
resistance rate of P. vulgaris to amoxycillin, ceftriaxone, ticarcillin, ciprofloxacin
and amikacin. The result revealed that the antimicrobial
resistance of P.
vulgaris was as follows, amoxycillin (100 %), ceftriaxon (33.3 %), ciprofloxacin
(0%), ticarcillin (0%) and amikacin (0 %).
Minimum inhibitory concentrations (MICs) for amoxicillin and ceftriaxon were
determine by E. test.
The result indicated that the MIC, MIC50 and MIC90 of amoxicillin was
(>240 μg/ml ), and ceftrixone were (0.01 and 0.1 μg/ml).
The study concluded the frequency of P. vulgaris in diabetic septic wounds was
slightly high.
The antimicrobial resistance to amoxicillin was high.
