Abstract:
This study was carried out in Khartoum state during the period from November
2008 to March 2009, to investigate antimicrobial resistance of Proteus mirabilis
isolated from patients suffering from community - acquired urinary tract infections.
Two hundred and nineteen urine specimens were collected from patients attending
Khartoum Teaching Hospital, Fudail Center, Gaffer Iben Auff specialized Hospital
for Children and Khartoum North Teaching Hospital.
The specimens were cultured on blood and MaCconkey's agars for primary
isolation of the pathogen. Identification of the isolates was done by colonial
morphology, gram stain and biochemical tests using API 20E.
Modified Kirby-Bauer disc diffusion method was adopted to determine the
resistance rate of P. mirabilis
to nitrofuratoin, amoxicillin, nalidixic acid, co-
trimoxazole, amoxyclav, ticarcillin, ciprofloxacin and ceftriaxone. Minimum
inhibitory concentrations (MICs) of ceftriaxone, amoxyclav, co-trimoxazole,
ciprofloxacin and chloramphenicol were determined by E. test.
Out of the two hundred and ninteen urine specimens examined, only 10 (4.6 %)
P. mirabilis were recovered.
The result revealed that the antimicrobial resistance of P. mirabilis was as follows:
amoxicillin and nitrofuratoin (100% each), co-trimoxazole (40 %), nalidixic acid,
Ciprofloxacin and Ceftriaxone (20.% each), and no resistant was observed to
amikacin, ticarcillin and amoxyclav.
The result indicated that the MIC, MIC50 and MIC90 of amoxyclav were (4 μg/ml,
each), co-trimoxazole (0.5 - 4 μg/ml, 0.5μg/ml and 4 μg/ml), ceftriaxone (0.008-
0.08μg/ml, 0.008μg/ml and 0.08 μg/ml) and ciprofloxacin (0.001- 0.01μg/ml,
0.001μg/ml and 0.0 μg/ml).
The study concluded that the responsibility of
P. mirabilis to UTIs slightly higher
than previously recorded. The antimicrobial resistance of P. mirabilis to
traditionally used antibiotics was also high.
