Abstract:
This study was carried out in Khartoum state during the period
from November 2008 to March 2009, to determine frequency of P.
stuartii in clinical specimens and its antimicrobial resistance. Four
hundred and twenty three urine and wound swabs (two specimens
from urine and three specimens from wound swabs) specimens were
collected from patients attended Khartoum Teaching Hospital,
Gaffer Iben Auff specialized Hospital for Children, Fedial Medical
Center and Omdurman Teaching Hospital.
The specimens were cultured on blood agar and MacConkey`s agar
for primary isolation pathogen . Identification of the isolates was
done by colonical morphology, gram stain and biochemical tests
using API 20E.
Modified Kirby – Bauer disc diffusion method was adopted to
detect the resistance rate of P. stuartii to nitrofuration, amoxycillin,
nalidixic acid, co-trimoxzole, amoxyclav, ticaricillin, ceftriaxone,
ciprofloxacin
and
amikacin.
Minimum
inhibitory
concentration
(MICs) to all antimicrobial agents were determine by E.test.
Of the four hundred and twenty three urine and swab specimens
examined, only 5(1.9%) P. stuartii were recovered. The result revealed
that the antimicrobial resistance of P. stuartii was as follows;
nitrofuration,
amoxycillin,
ticaricillin
were
(100% each) and
nalidixic acid, co-trimoxazole, amoxyclav, ceftriaxone, ciprofloxacin
and amikacin were (0% each).
The result indicated that the MIC, MIC50 and MIC90 of nitrofuration
(>240mg/ml each), co-trimoxzole (0.5 mg/ml each), amoxycillin (5-
120mg/ml, 60 mg/ml and 20 mg/ml), amoxyclav (30 mg/ml each),
nalidixic acid (0.05mg/ml each), amikacin (0.1-0.5mg/ml,0.5mg/ml
each) ciprofloxacin (7.5mg/ml each), ceftriaxone (0.25mg/ml each) and
ticaricillin (30-60 mg/ml and 60mg/ml each).
The study concluded that P. stuartii is one of the causative agent of
urinary tract infection and wounds infections.
