Abstract:
A new, precise and accurate reversed - phase high performance liquid chromatography(R-HPLC) a method was developed for determination of diazepam. High performance liquid chromatography was carried out by isocratic technique on a reverse-phase using column C18 inertstil (150 *3.9 mm), 5μm column with a mobile phase consisting of phosphate buffer solution pH = 6 and methanol in (1:1) ratio the diluents 5cm3 of sulfuric acid in 1000cm3 of methanol. The flow rate was adjusted at 2cm3/min, detection wavelength at 230nm, temperature at 40Cᵒ and retention time was found to be 6.4 min. The results of analysis have been validated statistically and recovery studies confirmed the accuracy of developed method according to ICH guidelines (ICH Q2 R1, 2005).The assay percentage of diazepam in tablet dosage form was found to be (98.61±0.89) %. The % of recovery was found to be (97.91-99.28) %. The limit of detection (LOD) and limit of quantization (LOQ) were found to be 0.000033mg/cm3 and 0.000101mg/cm3 respectively.
