Abstract:
Members of the family enterobacteriaceae- producing ESBLs constitute a serious threat
to current ß - lactam therapy. Moreover, in vitro detection of ESBLs expression has
proved to be difficult because many of these strains are reported susceptible to the widely
used and broad-spectrum ß-lactam antibiotics.
This study was carried out in Research Laboratory of Sudan University of Science and
Technology during the period from December 2009 to May 2010, to detect TEM, SHV
and CTX-M genes in ESBLs-producing Proteus vulgaris
Six strains of Proteus vulgaris were obtained from the Research Laboratory . The strains
were cultured on nutrient agar and examined microscopically to check their purity. DNA
extracted from each strain by boiling method. Multiplex PCR method was adopted to
detect gene present in Proteus vulgaris. The results showed that only TEM gene existed
in all tested strains. It is concluded the TEM is the common gene present in Proteus
vulgaris. further studies with large number of samples are required to validate the present
result .
