Abstract:
Beta- lactamases continue to be the leading cause of resistance to β-lactam antibiotics among
gram-negative bacteria. In recent years there has been an increased incidence and prevalence of
Extended-spectrum lactamases (ESBLs).This study was conducted in the Research Laboratory in
Sudan University of Science and Technology. The study was carried out during the period from
December 2009 to May 2010, to detect TEM, SHV and CTX-M genes in ESBLs-produsing
Klebsiella oxytoca.
The Klebseiella oxytoca strains were obtained from the Research Laboratory. All strains were
checked for purity by sub-culturing on nutrient agar and examined microscopically. Bacterial
DNA was extracted from each isolate using boiling method. Multiplex Polymerase chain
reaction (PCR) was adopted to detect these genes. The result revealed presence of TEM gene in
nine isolates. It is concluded that, TEM gene is the commonest gene in K. oxytoca isolates.
Further studies with large number of bacterial isolates are required to validate this results.
