Abstract:
This study was carried out in Khartoum state during the period from November to
May 2010 as extention to the previous research .The research aimed to
determine the frequency of Citrobacter frundii among patients with urinary tract
infection , also it aimed to determine the frequency of extended spectrum beta
lactamases ( ESBL) in this species by using modified Kirby–Bauer disc diffusion
method. Among three hundred and eleven ( n=311) patients, 11 (3,5%) were found
to have UTI caused by Citrobacter frundii among which eight (72,7%) were
considered as extended spectrum beta lactamases producer.
The research was extended to determine the most frequent gene which is
responsible for this phenomenon. Re-identification of the isolate was done by
colonial morphology. Gram,s stain and biochemical tests using API20E. Modified
Kirby– Bauer disc diffusion method was adopted to evaluate the resistance rate of
C. frundyii to penicillin and third generation cephalosporins.
Finally , DNA was extracted from each strain by boiling method and then PCR technique was
adopted to detect the presence of TEM gene in these isolates . The results showed that TEM
gene existed in all strains, these findings suggested that TEM gene may be the most dominant
factor that induce ESBLs phenomenon .
