Abstract:
Extended-spectrum ß-lactamases (ESBLs) have become widespread throughout
the world and are now found in a significant percentage of Citrobacter youngae
and other enterobacteriaceae strains in certain countries. This study was
conducted in the Research Laboratory in Sudan University of Science and
Technology. The study was carried out during the period from December 2009 to
May 2010, to detect TEM, SHV and CTX-M genes in ESBLs-producing C.
youngae.
Six C. youngae strains were obtained from the Research Laboratory. All strains
were checked for purity by sub-culturing on nutrient agar and examined
microscopically. Bacterial DNA was extracted from each isolate using boiling
method. Multiplex PCR was adopted to detect these genes. The result revealed the
presence of TEM gene only in four of the isolates. It is concluded that, TEM gene
is the commonest gene in C. youngae isolates. Further studies with large number
of bacterial isolates are required to evaluate this result.
