Abstract:
Extended-spectrum ß-lactamases (ESBLs) have become widespread throughout
the world and are now found in a significant percentage of Escherichia coli and
Klebsiella pneumoniae strains in certain countries. This study was conducted in
the Research Laboratory in Sudan University of Science and Technology. The
study was carried out during the period from December 2009 to May 2010, to
detect TEM, SHV and CTX-M genes in ESBLs-producing E. coli.
The E. coli strains were obtained from the Research Laboratory. All strains were
checked for purity by sub-culturing on nutrient agar and examined
microscopically. Bacterial DNA was extracted from each isolate using boiling
method. Multiplex PCR was adopted to detect the different genes including
(SHV, CTX-M). The result revealed presence of TEM gene only in six of the
isolates. It is concluded that, TEM gene is the commonest gene in E. coli isolates.
Thus, this gene may be the dominant one that responsible for ESBL phenomenon
among E. coli infection in Sudanese patients. Further studies required for
confirmation of presence of these genes in clinical Sudanese isolates.
