Abstract:
This retrospective study was conducted in histopathology laboratories in
Bahry hospital
and fideal hospital, and laboratory of Dr. Ishraga
Ahamed Fargalla, during the period from
This
study
aimed
to
detect
the
April to August 2011.
different
adenocarcinoma of the large
types
of
mucins
in
bowel. 67specimens from
adenocarcinoma of large bowel were stained
by six histopathological
special stains to detect types of mucins and to correlate histological
types and grade of the tumor with mucin type.the Haematoxylin and
Eosin (H&E) for general morphology, and periodic acid schiff's reagent
for neutral mucin and N-acetyl sialomucin(enzyme labile),variable for
sulfated and negative for O-acetylsialomucin(enzyme resistant),and
Phenylhydrazine-PAS stain which is negative for neutral mucin and
positive for other periodate-reactive mucins, and combined alcian blue-
PAS stain which is differentiated between acid and neutral mucin, and
combined aldehyde fuchsin-alcian blue stain to differentiate between
sulfated and carboxylated mucin, and alcian blue stain PH1for weakly
sulfated mucin.
The study concluded that: The type of mucin found in
this study, carboxylated sialomucin Oacetyle was the majority type 22
(32.80%), followed by carboxylated sialomucin N acetyl form 19
(28.40%), neutral mucin and carboxylated sialomucin were found in 12
(17.90%), and neutral and sulfated mucin in 6 (9.0%), while sulfated
mucin in 4 cases(6.0%) and neutral and acid mucin in 4 cases also.The
histological types of tumor are mucinous adenocarcinoma, non mucinous
adenocarcinoma
and
signet
ring
type,
distributed
as
9(13.4%),
52(77.6%), 6(9.0%) respectively. The histological grade of tumor
distributed as well differentiated 17(25.40%), moderately differentiated
38(56.70%)
and
poorly
differentiated
12(17.90%).
Finally
we
recommended conducting new studies contributing larger sample size and using more specific and sensitive techniques to identify different
.types and suptypes of mucins
