Abstract:
Diabetes mellitus is one of the most common endocrine metabolic
disorders, it has effects on various tissues of the body include the cells of
oral mucosa, and its glycogen content. One of the main objectives to
demonstrate the cytological changes and Periodic Acid Schiff's positivity
in the buccal mucosa of diabetes patient. This cross sectional descriptive
study conducted in Sudan University of Sciences and Technology, the
cytological sample were collected from diabetes patient attending,
Khartoum, Omdurman, and Bahry teaching hospitals, during the period
between February to May 2011. One hundred diabetic patients
approached to participate in this study, 30 non diabetes participated as
control. Two cytological smeared were prepared on two microscopical
slides and stained one with Papanicolaou stain and other with periodic
acid and Schiff reaction. The cytological examination of patient smears
showed that, 32 (32%), 36 (36%), and 32 (32%), had no change,
inflammatory reaction, and micro nuclear changes respectively. The
Periodic Acid Schiff's reactive results reported as fellows, 30 (30%), 46
(46%), 20 (20%), and 4 (4%), had negative, weekly positive, moderately
positive, and strongly positive respectively. While the control 24 (80%), 2
(6.7%) and 4 (13.3%) had no change, inflammatory reaction and micro
nuclear change respectively. All the Periodic Acid Schiff's reactive
results of the control group showed negative result. Comparison between
the cytological change and PAS, among the diabetics patient and control
group showed significant difference. The study conclude that the
Diabetes mellitus lead to cytological change in the buccal mucosa , and
this change found in diabetic duration from 11-20 year ,also the PAS
positivity in oral mucosa cells , these changes found in diabetic duration
less than 10 years. Further studies are needed to confirm this finding with
larger sample size.
