Abstract:
This is an analytical case –control study, conducted at Al–Gdarif teaching
hospital, during the period from January to April 2011. The aim of this study was
assessment of the hematological parameters (complete blood count), and serum
ferritin, tests in visceral Leishmaniasis (v l) patients, attended Al-Gdarif teaching
hospital. Fifty visceral Leishmaniasis patients were informed about the study and
agreed for participation .The study population (both sex) was divided into three
group according to age .
Five ml of venous blood was bleed ,from both patients and control , 2.5ml in
EDTA( 1.2mg/ml) containers and 2.5ml without anticoagulant (serum) ,to
investigated the CBC ,peripheral blood film ,reticulocyte count, and serum-ferritin
tests .Fully automated hematological analyzer sysmex was used for CBC, and
manual analysis for blood film ,and reticulocyte count ,and a fully automated
immunoassay system (AIA -600 11) was used for Serum-ferritin analysis. The
staining films( Ral-stain), were examined to detect the peripheral blood
morphology, and other staining films(vital stain) to count reticulocyte cell
manually .
All visceral Leishmaniasis patients had mean value of HB( 7.2g/dl)
,RBC(3.6×106), Hct( 25.4%), MCV(69.5 fl), MCH(19.9 pg), MCHC(28.1g/dl),
WBCs(3.2×106μl), Neutrophil(33.2%) ,And platelet(142.2×10 3μl), decrease, highly
significant than control mean values( All p value=0.00).The mean of lymphocyte,
and eosinophil,( 50.8% ,11.2%,respectively), significant increase than control
mean values (p value=0.00) due to infecation. The mean of the Serum-ferritin in
test (33.2 ng/ml), showed significant decrease than control (52.7 ng/ml) ,but with
normal rang .The reticulocyte count showed significant increase in test ,than
control (mean=4.8%).
