Abstract:
As chornic gastritis and peptic ulcer diseases, particularly duodenal ulcer,
are the most common endoscopic findings in Sudanese dyspeptic patients, it
was necessary to study the association between H. pylori infection and
gastro- duodenal diseases in developing countries such as Sudan.
Material and Methods: One hundred and twenty subjects referred to
Gastroentrology Department, Ibn Sina Hospital, Khartoum were studied.
Most of the patients were referred because of dyspepsia. After endoscopic
examination 97 were found to have definite pathology and 23 were found to
have normal endoscopic examination. Age range from 18 to 80 years old. .
Male to female e sex ratio was (2:1).
Endoscopic biopsies were collected from all subjects. In this study, culture of
H. pylori was used as the gold standard method to which histological (urease)
.test, was compared as alternative diagnostic technique
Results:. 93 subject were H. pylori positive when using culture. The CLO test
detected 85 H. pylori positive and 35 were negative .
H. pylori was found not to be associated (p>0.05) with age, sex,
cigarette smoking urease test, socio-economic status, residence, Tribe and
water resources disposable system
However, it is assocciated (p< 0.05) with complaints (epigastric pain,
abdominal pain and vomiting), endoscopy finding (gastric ulcer, duodenal
ulcer, gastritis and duodenitis) and the presence of 120 kda.
The protein pattern of H. pylori when analyzed with sodium deodecylsulphate
polyacrylamide gel electrophoresis (SDS-PAGE) technique are not markedly
different between strains of different origin SDS-PAGE The major protein
bands with molecular weights of 120, 94, 88,75, 69, 67, 61, 48, 45, 42, 30,and
25Kda were variably present.
IV
The data from this study proves the high correlation of 120 Kda protein
to endoscopic findings (x2 = 16.047 p value = 0.003 p < 0.05 highly
significant. In this study 57 (61%) out 93 H. pylori strains that were isolated
from 120 subjects possess the gene that expressed its 120 Kda product. The
120Kda was detected in H. pylori isolated from one subject with normal
endoscopy , 11(11.8%) with duodenal ulcer, 8( 8.6) patient with gastric ulcer,
23(23.7%) patient with gastritis, 14 (15.1%) patients with duodenitis and only
1 patient with oesophagitis The present study confirms a high prevalence of
H. pylori infection in Sudanese patients as in most population in Africa and
other developing countries.
