Abstract:
The objectives of this study was to evaluate the yield and purity of DNA
extracted from stored and fresh blood samples.
Analytical, comparative study was conducted in Khartoum state during
the period from January to June, 2011.This study included 70
subjects, 35 of them were fresh blood samples and 35 of them
were stored for three years.
Two and half ml of whole blood was collected in ethylene di-amine tetra
acetic acid (EDTA) containers so as to determine the total white
blood cells count by Glacial acetic acid and to extract the
genomic DNA by chelex method. the DNA concentration was
measured by spectrophotometer (Biophotometer, eppendorf) at
260 nm, DNA purity was determined by the A260/A 280 ratio.
The results showed that the mean concentration of DNA extracted from
fresh blood samples was (220.6μ g/mL) ,which slightly higher than that
extracted from stored blood samples (207.7μ g/mL), (P=0.14). The study
also showed that the mean ratio of DNA extracted from fresh blood
samples (1.06) was similar to stored ones (1.06), (P=0.64). Also in this
study showed a weak correlation between TWBCs and the DNA
concentration in fresh blood sample (r-0.08) and (P=0.64).
