Abstract:
This analytical, descriptive and cross-sectional study aim to evaluate the
hematological parameters of sickle cell disease and sickle cell trait gene
patients compared to control individuals admitted to Omdurman Pediatric
hospital of Emergency in Khartoum state in the period between April 2011 to
July 2011.
One hundred (100) patients, and(50)controls were informed their parents
about consent for participation was obtained. A venous blood of 2.5ml was
collected in ethylene-diamine-tetra-acetic acid (EDTA) containers and
investigated for sickle cell disease and sickle cell trait, a complete blood count
(CBC) [semi automated hematological analyzer (Sysmex kx21N)]was used,
also blood film, correction of WBCs, reticulocyte count, and hemoglobin
electrophoresis were carried out, and statistical package for social sciences
(SPSS) computer program version 16 was used for data processing.
The percentage of HbAA according to the gender (70%) in the males and
(30%) in the females, HbAS ( 20%) of the males (16%) of the females.
HbSS (33%) and(28%) in the males and females respectively. HbSF (2%) of
the males and (1%) of the females.
The percentage of Hb type according to the age (30%) of HbAA were <5
years, (36%) were between 5 and 10 years and (34%) were >10 years. For
HbAS (11%) were <5 years, (15%) were between 5 and 10 years and (10%)
were > 10 years. 27% of HbSS were <5 years, 25% were between 5 and 10
years and 9% were >10 years. All the frequency of Hb SF (3%) were found to
be <5 year.
The means of Hb, RBCs & PCV were significantly increase in Hb AA than in
Hb SS while the means of platelets count, reticulocytes count, & total
leukocyte counts were significantly decrease in Hb AA than in Hb ASS.
There were found significant increase in Hb AS than Hb AA in the neutrophil
count with the p value (< 0.05) and mixed with the p value(<0.05). Other
parameters show no difference between Hb AS and Hb AA.
This results showed that means of Hb, RBCs & PCV were significantly
decrease in Hb SF than in Hb AA while the means of PLTs count, absolute
neutrophil and mixed were significantly increase in Hb SF than in Hb AA.
The means of Hb, RBCs & PCV were significantly decrease in Hb SS than in
Hb AS while the means of platelets count, reticulocytes count, & total
leukocyte count were significantly increase in Hb SS than in Hb AS.
