Abstract:
This study was carried out in Ahmed Gassim Hospital, Khartoum State during the
period from April to June 2012. A total of 100 mid-stream urine specimens were
collected, they were cultured on Cystiene Lysine Electrolytes Deficiency (CLED)
agar. The identification of uro-pathogens was done according to Chessbrough
(2007) which involved colonial morphology, Gram stain and biochemical
identification, using catalase test, coagulase test, DNase test, mannitol salt agar,
indole test, urease test, citrate test, MR test, VP test, motility test, and kligler iron
agar.
Modified Kirby ـBauer disc diffusion method was used to study the susceptibility
patterns of isolated bacteria against the following antibiotics: amikacin, ampicillin,
cotrimoxazole,
ceftizoxime,
cefotaxime,
chloramphenicol,
ciprofloxacin,
gatifloxacin, cephalexin, sparfloxcin, norfloxacin and tetracycline.
56(56%) from all urine culture were showed significant bacterial growth and 44
(44%) showed no or insignificant growth. According to the scheme of
Chessbrough) 2007(, six different type of bacteria were isolated and identified, the
most dominant species was Escherichia coli 23 (41%), followed by
Staphylococcus aureus21 (37.5%), Proteus spp. 5(9%), Klebsiella spp. 3(5.3%),
Staphylococcus epidermidis and Pseudomonas spp. were represented2 (3.6%). All
isolates 6 (100%) were resistant to ceftizoxime. and most of them 5(83%) resistant
to cephalexin. All isolates 6 (100%) were sensitive to amikacin, chloramphenicol,
and 5(83%) of them were sensitive to gatifoxacin while tetracycline and
sparfloxcin were sensitive only to Staphylococcus spp. the result showed that the
E.coli and Pseudomonas spp. were resistant to the most used antibiotic, they were
sensitive only for 4 (30%) agents out of 12 used agents.
This study showed that Escherichia coli is a most common
causative agent of the urinary tract infection among
haemodialysis patients, and Amikacin is the most susceptible
antibiotic pattern.
