Abstract:
Nosocomial Multi-drug Resistant Gram-negative bacterial infections are one
of serious health problems worldwide.
The objective of this study was to isolate, identify and assess nosocomial
multi-drug resistant Gram-negative bacteria in infected wounds
One hundred clinical swap samples were collected from patients with
infected wounds. The identification of the isolated organisms utilized
cultural characteristics, Gram’s stain, and biochemical tests. Antimicrobial
susceptibility testing was carried out by disk diffusion method on Mueller
Hinton agar medium by Kirby Bauer technique.
Out of the total number of specimens investigated, 37 of them showed no
growth. 38 isolates were Gram positive cocci and 25 isolates were Gram
negative bacilli.
From the 25 isolates identified as gram negative bacilli, 17 isolates were P.
aeruginosa, 4 isolates were E. coli, 2 isolates were K. pneumoniae and 2
isolates were P. vulgaris. 23 isolates were sensitive to all antibiotic disks,
while two isolates were multi-drug resistant.
The study concluded that P. aeroginosae and E. coli were the most common
Gram- negative pathogens isolated from the nosocomial infections in wound
infected patients. Further studies are needed to confirm the present results.
