Abstract:
Gastroenteritis
is
one
of
the
serious
health
problems
worldwide. Escherichia coli is the leading organism among the causative
agents of gastroenteritis.
The objective of this study was to isolate, identify and serotype
Escherichia coli from patients with gastroenteritis.
A total of 100 stool specimens were collected from patients
attended Military Hospital and
Hospital
For
Pediatric,
Mohammed Alamen Hamed
Omdorman.
The
specimens
were
cultivated on bacteriological culture media for primary isolation
of the pathogen. E. coli was identified by Gram's stain and
biochemical tests. Serotyping for compliment cultural findings
was done by slide agglutination of heat treated organisms
method to detect enteropathogenic E. coli.
The result revealed that 87 (87% ) E. coli were isolated from
the hundred specimens. 33 (38% ) of the isolates were EPEC.
Out of them, 23 (70% ) of these were poly 2-serogroup and 10
(30% ) were poly 3-serogroup.
The study concluded that EPEC serotype was the main
causative agent of gastroentritis in children than in adult.
Further studies are needed to confirm the present result as
well as using monovalent antisera for specific serotyping.
