Abstract:
This study was designed to investigate the presence of Pseudomonas
aeruginosa among patients with different diseases and to subject the
isolated P. aeruginosa to molecular characterization and antibiotic
susceptibility patterns in Khartoum state.
The study was performed during the period from February to June 2011.
It included 150 samples (different types of bacteria were isolated
including P. aeruginosa 74 (49.3%) and others 76 (50.7%)) from urine,
wounds, eyes, ears, vaginal infections, sputum, shunts, pleural fluid
aspirates and cerepro spinal fluid and asitic fluid. This samples were
collected and examined in Khartoum Teaching Hospital and the research
laboratory at the Sudan University of Science and Technology (SUST).
These were collected from patients with different sexes and ages. The
samples were cultured on cystine lactose electrolyte deficient agar
(CLED), MacConkey agar, blood and chocolate agar, incubated
aerobically at 37oC. Gram stain was done for bacterial isolates.
Biochemical tests were used to identify isolated organism (bacteria).
Susceptibility tests were designed to determine the antibiotic resistance of
isolated P. aeruginosa. Molecular characterization (polymerase chain
reaction "PCR") amplification target genes IMP-7 and IMP-10 were done
for isolated P. aeruginosa.
The study revealed that P. aeruginosa was isolated from samples was
(49.3%) and others (50.7%) were resistant to (20) carbenicillin (CB)
percentage was (13.3%).
The antibiotic resistance tests showed that (74) of P. aeruginosa isolates
(49.3%) were resistant to carbenicillin.
DNA extraction by Boiling-centrifugation method from culture were
done for (57) isolates, PCR showed (2) isolates (26) (45.6%) were IMP-7
primer reorganized and (31) isolates (54.4) were not IMP-7 primer, also
PCR showed (2) isolates (31) (54.4) were IMP-10 primer recognized and
(26) (45.6%) were not IMP-10 primer.
This study showed that the identification of P. aeruginosa by molecular
characterization (PCR) and sensitivity patterns among patients with
different diseases in Khartoum state. Amplification of IMP-10 gene was
more active than IMP-7 gene which is desirable due to the unlimited of
this genes in the strains of P. aeruginosa.
