Abstract:
Worldwide diarrheal diseases are the second leading
cause of death; about 25 million enteric infections occur
each year.
One hundred faeces samples were collected from
patients attended to Alribat, Al Raed Al Salam and Military
Hospital during April to June 2012. The samples were
examined
macroscopically
and
microscopically
then
cultured on CHROM agar and MacConkey agar for primary
isolation of some aerobic pathogens. Purification of
isolates was carried out on Nutrient agar.
The results revealed high percentage of mixed
isolates 29 (29 %) on CHROM agar compared with
MacConky agar 13 (13 %).
The isolated Gram-negative bacteria were 114
(87.02%) while Gram-positive bacteria were 17 (12.97%)
on CHROM agar.
The isolated Gram-negative bacteria were
Escherichia coli 81 (61.83%), Shigella dysenteriae 17
(12.97%), Klebsiella aerogenes 9 (6.87%), Salmonella
typhi 2 (1.52%), Salmonella typhimurium 1 (.76%),
Klebsiella ozanae 1 (.76%), Klebsiella oxytoca 1 (.76%),
Citrobacter diversus 1 (.76%) and Proteus mirabilis 1
(.76%) while Gram-positive bacteria were Staphylococcus
aureus 9 (6.87%) and Streptococcus faecalis 8 (6.10%).
It was concluded that CHROM agar was a very
helpful medium for primary isolation of some aerobic pathogens from faeces specimen especially in mixed
.infection
