Abstract:
This is a descriptive retrospective study carried out in central laboratory-Sudan
during the period between February 2012 to July 2012.The study aimed at
detection of the association of Epstein-Barr virus with oesophageal and stomach
cancers among Sudanese Patients using Polymerase Chain Reaction. Samples from
39 patients were included (21 with stomach cancer and 18 with esophagus cancer)
their ages ranging from 29 to 70 years with mean age of 55 years old.
All patients underwent upper gastrointestinal endoscopy, two Biopsies were taken,
one of them was immediately fixed in 10% formalin and processed by
conventional methods for paraffin wax embedding and H&E stain to verify an
adequate number of stomach or esophagus cancer cells were present, other one was
collected in normal saline to extract the DNA for detection of EBNA-1 gene of
EBV using PCR. SPSS version 16 computer program was used to analyze the data
and results.
Out of 39 patients with upper GIT cancer, 22(56.4%) patients were males and
17(43.6%) patients were females. EBV expressed in 2(5.1%) of males and
5(12.8%) of females. 21 (53.8%) were stomach cancer, EBV was positive among 5
of the cases (12.8%), 18 (46.2%) of the demonstrated samples were diagnosed as
esophagus cancer , only 2 samples (5.1%) showed positivity for EBV.
On the basis of these findings the study concluded that; the expression of EBV in
upper GIT malignancies is statistically insignificant P. value = 0.303.
