Abstract:
This study was carried out to study the effect of cold shock on certain organisms
which routinely isolated from a variety of clinical specimens, Escherichia coli,
Enterococcus faecalis,and Salmonella typhi on their growth and evaluation and
compression of minimum inhibitory concentration of these organisms with
amoxicillin, cephalexin, ceftriaxone, ciprofloxacin, chloramphinicol and co-
trimoxazole antibiotics before and after cold shock in -20c for at 1 hour.
This study revealed that clinical assessment showed that Eschericia coli before
cold shock at -6 dilution gave a result 1.8 x 10 8CFU/ml, at -7 dilution gave 0.5
x109CFU/ml, at -8 dilution gave 0.4 x1010 CFU/ml, and after cold shock the result
was 0.8 x108 in -6 dilution and at -7 dilution gave 0.3 x109CFU\ml and at -8 is
0.2x1010 CFU\ml. The result for Salmonella typhi before cold shock in -6, -7, -8,
and -9 gave uncounted colonies, and at -10 gave 4.1 x10 12 CFU\ml, and after cold
shock, at -6 dilution produced 4.4 x108, at -7 dilution produced 3.1 x109CFU/ml,
and at -8 dilution produce 1.6 x1010CFU/ml, and at -9 and -10 dilution gave no
growth. Enterococcus faecalisbefore cold shock in -6 tube dilution and -7 dilution
the result is uncounted colonies, at -8 dilution it gave 2.05 x10 11CFU/ml, at -9
produce 1.68 x1012and at -10 7.8 x1012CFU/ml. After cold shock at -6 uncounted,
at -7 gave 1.63 x1010, at -8 gave 1.33 x1011CFU/ml and at -9 dilution gave 1.02
x1012 and -10 gave 3.9 x1012
Result of minimum inhibitory concentration for Escherichia coli before cold shock
is resistant to amoxicillin, cephalexine and co-trimoxazole, the result with
ceftriaxone is 240 mcg, Ciprofloxacin 7.5 mcg, chloramphinicol 1 mcg. After cold
shock Amoxicillin, cephalexin, ceftriaxone are resistant, ciprofloxacin 3mcg,
chloramphinicol 1mcg. For Salmonella typhi before shock Amoxicillin, co-
trimoxazole and ciprofloxacin are resistant, cefalexine 30mcg, ceftriaxone 0.008
mcg, chloramphinicol 0.1 mcg, and co-trimoxazole 0.1 mcg. After cold shock
amoxicillin, cephalexin and ciprofloxacin are resistant, ceftriaxone 0.008mcg,
chloramphinicol 0.1 mcg and co-trimoxazole 0.05 mcg. For Enterococcus faecalis
before cold shock with amoxicillin 4 mcg, cefalexine 3 mcg, ceftriaxone 0.01 mcg,
ciprofloxacin 10 mcg, chloramphinicol 0.5 mcg, and co-trimoxazole 0.1 mcg. After
cold shock amoxicillin 4 mcg, cephalexin 3 mcg, ceftriaxone 0.01 mcg,
ciprofloxacin 10 mcg, chloramphinicol 0.5 mcg and co-trimoxazole 0.1 mcg.
