Abstract:
Research for antibiotics has been widely performed for about last 50
years and new antibiotics are still being found. This study was
carried out during the period from March to June 2011, to
isolate
antibiotic-producing bacteria from soil. Soil samples were collected in
sterile plastic bag then cultured on nutrient agar enriched with soil
extract. Antibiotic- producing bacteria were detected by plate over-
lay method using standard bacteria as indicator. Activity of antibiotic
was evaluated against standard bacterial strains and clinical
isolates. The result showed that different promising organisms were
isolated. Of the broad spectrum antibiotic-producers, one isolate
was choosen and identified as Bacillus megaterium. The product
was found to be active against E. coli and Citrobacter species in 5%
glucose concentration. It is concluded that sudanese soil teems with
antibiotic producers and further investigations are required to
validate this result.
